Database : HANSEN Search on : SENSIBILIDADE E ESPECIFICIDADE [Subject descriptor] References found : 48 [refine] Displaying: 1 .. 20   in format [Detailed]

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Id: 17565 Author: Patrocinio, Lucas Gomes; Goulart, Isabela Maria Bernardes; Goulart, Luiz Ricardo; Patrocinio, Jose Aantonio; Ferreira, Frederico Rogerio; Fleury, Raul Negrao Title: Detection of Mycobacterium leprae in nasal mucosa biopsies by the polymerase chain reaction ..- Source: s.l; s.n; 2005. 6 p. ilus, tab. Abstract: Several discoveries about leprosy indicate that Mycobacterium leprae transmission mainly occurs by inhalation, and the nose is major port of entry and exit. The present study evaluated the clinical application of PCR for detection of M. leprae DNA in nasal mucosa biopsies in untreated leprosy patients (52) and their contacts (99) from the State Reference Center in Sanitary Dermatology and Leprosy, Uberlandia, MG, Brazil. PCR detection of a 372-base pair DNA fragment from M. leprae was accomplished in 36 (69.2%) patients, from which 34 (91.9%) of them were multibacillaries. Furthermore, PCR was positive in 3 (16.7%) of 18 slit-skin smear negative, 4 (25.0%) of 16 skin lesion BI negative, 8 (33.3%) of 24 nasal mucosa BI negative patients, and 10 of 99 contacts (10.1%). The presence of bacilli in 10.1% of the contacts may potentially reflect an occult leprosy, and these patients must be accompanied, followed by a chemoprophylaxy treatment. Considering all PCR results against clinical and BI classification of patients and controls, we have found a sensitivity of 69.2%, a specificity of 89.9%, and an accuracy of 82.8%. It has been demonstrated here through PCR of nasal biopsies that the bacillus invades the mucosa, passing through the nasal inferior turbinate to reach peripheral blood. Therefore, the molecular investigation of invasive nasal biopsies by PCR tests has proven to be useful in defining patients of higher risk of transmission and risk-group contacts, which is an important step to reach the World Health Organization objective towards the elimination of leprosy as a public health problem. (AU). Descriptors: Biópsia Brasil/EP DNA Bacteriano/AN Hanseníase/*DI/EP/MI Mycobacterium leprae/GE/*IP Mucosa Nasal/*MI/PA Reação em Cadeia da Polimerase/* Fatores de Risco Sensibilidade e Especificidade Limits: Estudo Comparativo Humanos Location: BR191.1; 09345/s

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Id: 17564 Author: Kramme, Stefanie; Bretzel, Gisela; Panning, Marcus; Kawuma, Joseph; Drosten, Christian Title: Detection and quantification of Mycobacterium leprae in tissue samples by real-time PCR ..- Source: s.l; s.n; 2004. 5 p. tab, graf. Abstract: Real-time PCR technology has improved molecular diagnostics of many pathogens, but no such test is available for Mycobacterium leprae. In this report we describe the establishment and the pre-clinical evaluation of such an assay. The test achieved a theoretical analytical sensitivity limit of 194 M. leprae cells per skin biopsy specimen and facilitated quantification of mycobacteria in tissue over a range of 54-54,000,000 cells per sample. In punch skin biopsies from 39 untreated Ugandan patients with newly diagnosed leprosy, the clinical diagnosis could be confirmed in 88.9% of multibacillary and 33.3% of paucibacillary (microscopically negative) patients. Real-time detection thus did not increase the clinical sensitivity of PCR as compared to conventional protocols, in spite of its evidently high analytical sensitivity. On the other hand, as still no culture system exists for M. leprae, the assay appears to be a robust tool for detection of the bacterium in selected clinical situations, as well as for quantitation in experimental settings. (AU). Descriptors: Antígenos de Bactérias/GE Proteínas de Bactérias/GE Biópsia DNA Bacteriano/CH/IP Hanseníase/*DI/MI Dados de Sequência Molecular Mycobacterium leprae/GE/*IP Reação em Cadeia da Polimerase/*MT Sensibilidade e Especificidade Alinhamento de Sequência Análise de Sequência de DNA Pele/MI Uganda Limits: Research Support, Non-U.S. Gov't Humanos Location: BR191.1; 09344/s

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Id: 17312 Author: Kalk, Andreas; Konig, Jurgen; Van Wijnen, Ary; Wiegandt, Axel Title: Drug packaging: what can TB learn from leprosy control? ..- Source: s.l; s.n; 2003. 1 p. . Descriptors: Antituberculosos/*PD/TU Controle de Doenças Transmissíveis/* Sensibilidade e Especificidade Tuberculose Pulmonar/DI/*DT Limits: Estudo Comparativo Embalagem de Medicamentos/* Feminino Humano Masculino Location: BR191.1; 01753/s

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Id: 17303 Author: Queiroz, R. H. C; Pereira, R. C; Gotardo, M. A; Cordeiro, D. S; Melchior, E Title: Determination of clofazimine in leprosy patients by high-performance liquid chromatography ..- Source: s.l; s.n; 2003. 4 p. ilus, tab. Abstract: An original, simple, specific, and rapid high-performance liquid chromatography assay for the determination of clofazimine in human plasma is presented. The procedure consists of extracting the drug and the internal standard (medazepam) from 0.5 mL plasma with dichloromethane/diisopropyl ether (1:1, v/v) at pH 3.0, after precipitating the proteins with methanol. The drugs were then quantitated on a reversed-phase C8 using a mobile phase consisting of a mixture of methanol/0.25 N sodium acetate buffer at pH 3.0 (74:26, v/v). The flow-rate and wavelength were set at 1 mL/min and 286 nm, respectively. The precision, linearity, and limit of quantitation of the method were within acceptable limits. The method was considered adequate and could be applied in studies involving blood level monitoring and pharmacokinetics in leprosy patients. (AU). Descriptors: Cromatografia Líquida de Alta Pressão/*MT Clofazimina/*PK/TU Monitoramento de Medicamentos/*MT Hansenostáticos/*PK/TU Hanseníase/DT/*ME Reprodutibilidade de Resultados Sensibilidade e Especificidade Limits: Feminino Humano Masculino Location: BR191.1; 01158/s

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Id: 17276 Author: Qinxue, Wu; Xinyu, Li; Wei, Hou; Tao, Li; Yaoping, Ying; Jinping, Zhang; Xiuling, Cai; Ganyun, Ye Title: A study on PCR for detecting infection with M. leprae ..- Source: s.l; s.n; 1999. 5 p. tab. Abstract: OBJECTIVE: So far, it has not been established a satisfactory method for early diagnosis and studying on epidemiology for leprosy, we want to develop a molecular biological method for solving this point. MATERIALS AND METHODS: Based on the M. leprae gene coding groEL, 65 kD and 16S rRNA, three polymerase chain reactions were developed by using Plikaytis', Woods' and Pattyn's procedures. It was optimized that the experimental parameters for each PCR, and a comparative study on practivity among three PCRs was also conducted for practical purpose. RESULTS AND CONCLUSION: For detecting infection with M. leprae, all of PCRs established by us were highly sensitive and specific, but for practical purpose, the Woods' PCR optimized by us ought to be chosen firstly. (AU). Descriptors: Estudo Comparativo Primers do DNA DNA Bacteriano/GE Proteína GroEL/GE Hanseníase/*DI/GE/MI Mycobacterium leprae/*GE Mycobacterium tuberculosis/GE Reação em Cadeia da Polimerase/*MT RNA Ribossômico 16S/GE Sensibilidade e Especificidade Limits: Humano Location: BR191.1; 09175/s

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Id: 13625 Author: Antunes, Abílio; Nina, Jaime; David, Suzana Title: Serological screening for tuberculosis in the community: an evaluation of the Mycodot procedure in an African population with high HIV-2 prevalence (Republic of Guinea-Bissau) ..- Source: s.l; s.n; 2002. 5 p. tab. Abstract: The immuno-dot-blot assay MycoDot, which detects lipoarabinomannan (LAM) antibodies, was evaluated for the serological diagnosis of active pulmonary tuberculosis in patients in a rural community in the Republic of Guinea-Bissau. Sera from 269 adults (age > 15) and 33 children (age < 5) were assayed for antibodies in a blind manner and the results compared to the clinical status of tuberculosis. The assay had a specificity and a sensitivity of 92.4 per cent and 63.0 per cent respectively, when applied to the adult population. In HIV-2 infected individuals (27/269), the specificity and sensitivity of the assay were similar, 94.7 per cent and 62.5 per cent respectively. The assay did not provide high sensitivity for the diagnosis of tuberculosis in children. Sera from patients with leprosy cross-reacted with the antigen of the assay. It is concluded that this easily performed assay may be useful for the presumptive diagnosis of tuberculosis in adult populations in rural areas of developing countries where routine screening is not readily available. (AU). Descriptors: ANTICORPOS ANTIBACTERIAS/sangue GUINE BISSAU INFECCOES POR HIV/compl HIV-2 IMMUNOBLOTTING/métodos LIPOPOLISSACARIDIOS/uso diag POPULACAO RURAL MYCOBACTERIUM TUBERCULOSIS/isol SENSIBILIDADE E ESPECIFICIDADE TUBERCULOSE/compl TUBERCULOSE/diag TUBERCULOSE/patol TUBERCULOSE/virol Limits: ESTUDO COMPARATIVO HUMANO PRÉ-ESCOLAR CRIANÇA ADULTO SUPPORT, NON-U.S. GOV'T ADOLESCENTE Electronic Medium: http://www.ilsl.br Location: BR191.1; 09016/s

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Id: 13214 Author: Srinivasan, H; Stumpe, B Title: Leprosy diagnosis: a device for testing the thermal sensibility of skin lesions in the field ..- Source: s.l; s.n; 1989. 7 p. ilus, tab, graf. Abstract: A handy device for testing the thermal sensibility of skin lesions has been developed and field tested in various centres in Africa and India. The instrument performed satisfactorily and its use made testing for thermal sensibility in the field practicable and straightforward. Analysis of the results of testing 260 persons, most of whom exhibited a few lesions that were characteristic of early leprosy, showed that the rate of diagnosis of sensory impairment of such skin lesions, and hence the diagnosis of leprosy, would be about 15-25% more if thermal sensibility testing using this device were added to the other tests of sensibility routinely carried out in the field. Regular use of the device in the field would help to bring more leprosy patients under treatment than at present.(AU). Descriptors: HANSENIASE/diag HANSENIASE/fisiopatol PROJETOS PILOTO CALOR AFRICA SENSACAO SENSIBILIDADE E ESPECIFICIDADE PELE/inerv Limits: HUMANO SUPPORT, NON-U.S. GOV'T Electronic Medium: http://www.ilsl.br Location: BR191.1; 02112/s

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Id: 12293 Author: Boldsen, Jesper L Title: Epidemiological approach to the paleopathological diagnosis of leprosy ..- Source: s.l; s.n; 2001. 8 p. tab, mapa. Descriptors: ANTROPOLOGIA FISICA OSSO E OSSOS FOSSEIS HANSENIASE HANSENIASE PREVALENCIA VALORES DE REFERENCIA SENSIBILIDADE E ESPECIFICIDADE Location: BR191.1; 08671/s

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Id: 12248 Author: Williams, Diana L; Pittman, Tana L; Gillis, Thomas P; Matsuoka, Masanori; Kashiwabara, Yoshiko Title: Simultaneous detection of mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis ..- Source: s.l; s.n; Jun. 2001. 6 p. ilus, tab. Descriptors: SEQUENCIA DE BASES DNA BACTERIANO DNA BACTERIANO DAPSONA RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE ANALISE DE SEQUENCIA DE DNA Análise Heteroduplex/*MT Location: BR191.1; 08678/s; BR191.1; 08430/s

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Id: 12230 Author: Salotra, P; Sreenivas, G; Nasim, A. A; Subba Raju, B. V; Ramesh, V Title: Evaluation of enzyme-linked immunosorbent assay for diagnosis of post-kala-azar dermal leishmaniasis with crude or recombinant k39 antigen ..- Source: s.l; s.n; Mar. 2002. 4 p. tab, graf. Descriptors: ANTICORPOS ANTIPROTOZOARIOS ELISA ESTUDOS DE AVALIAÇAO LEISHMANIA DONOVANI LEISHMANIA DONOVANI LEISHMANIA DONOVANI LEISHMANIOSE VISCERAL LEISHMANIOSE VISCERAL PROTEINAS DE PROTOZOARIOS SENSIBILIDADE E ESPECIFICIDADE Limits: ANIMAL Location: BR191.1; 08713/s

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Id: 11936 Author: Mujagic, Hamza; Chabner, Bruce A; Mujagic, Zlata Title: Mechanisms of action and potential therapeutic uses of thalidomide ..- Source: s.l; s.n; 2002. 12 p. tab. Descriptors: MIELOMA MULTIPLO MIELOMA MULTIPLO NEOPLASIAS NEOPLASIAS DEFEITOS DO TUBO NEURAL DEFEITOS DO TUBO NEURAL SENSIBILIDADE E ESPECIFICIDADE RELAÇAO ESTRUTURA-ATIVIDADE TALIDOMIDA TALIDOMIDA TALIDOMIDA RESULTADO DE TRATAMENTO Location: BR191.1; 08595/s

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Id: 11824 Author: Mujagic, Hamza; Chabner, Bruce A; Mujagic, Zlata Title: Mechanisms of action and potential therapeutic uses of thalidomide ..- Source: s.l; s.n; 2002. 12 p. ilus, tab. Descriptors: ENSAIOS CLINICOS MIELOMA MULTIPLO MIELOMA MULTIPLO NEOPLASIAS NEOPLASIAS SENSIBILIDADE E ESPECIFICIDADE TALIDOMIDA TALIDOMIDA TALIDOMIDA Location: BR191.1; 08562/s

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Id: 11729 Author: Nishimura, Masayuki; Kwon, Kyung Sool; Shibuta, Kenji; Yoshikawa, Yasuji; Oh, Chang-Keun; Suzuki, Tomokazu; Chung, Tae-Ahn; Hori, Yoshiaki Title: An improved method for DNA diagnosis of leprosy using formaldehyde-fixed, paraffin-embedded skin biopsies ..- Source: s.l; s.n; 1994. 4 p. ilus, tab. Descriptors: HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE SEQUENCIA DE BASES DNA BACTERIANO ELETROFORESE EM GEL DE AGAR FORMALDEIDO DADOS DE SEQUENCIA MOLECULAR INCLUSAO EM PARAFINA REAÇAO EM CADEIA POR POLIMERASE SEQUENCIAS REGULADORAS DE ACIDO NUCLÉICO SENSIBILIDADE E ESPECIFICIDADE PELE FIXAÇAO DE TECIDOS Location: BR191.1; 07218/s

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Id: 11723 Author: Boldsen, Jesper L Title: Epidemiological approach to the paleopathological diagnosis of leprosy ..- Source: s.l; s.n; 2001. 8 p. tab, mapas. Descriptors: HANSENIASE HANSENIASE ANTROPOLOGIA FISICA OSSO E OSSOS FOSSEIS PREVALENCIA VALORES DE REFERENCIA SENSIBILIDADE E ESPECIFICIDADE ESTUDOS EPIDEMIOLOGICOS Limits: HUMANO ADULTO FEMININO Location: BR191.1; 07356/s

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Id: 11645 Author: Hailu, Asrat Title: The use of direct agglutination test (DAT) in serological diagnosis of Ethiopian cutaneous leishmaniasis ..- Source: s.l; s.n; 2002. 6 p. tab, graf. Descriptors: LEISHMANIA LEISHMANIOSE CUTANEA LEISHMANIOSE CUTANEA LEISHMANIOSE CUTANEA TESTES DE AGLUTINAÇAO ANTICORPOS ANTIPROTOZOARIOS BIOPSIA HISTOCITOQUIMICA SENSIBILIDADE E ESPECIFICIDADE PELE PELE Location: BR191.1; 08506/s

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Id: 11644 Author: Mujagic, Hamza; Chabner, Bruce A; Mujagic, Zlata Title: Mechanisms of action and potential therapeutic uses of thalidomide ..- Source: s.l; s.n; 2002. 12 p. tab. Descriptors: TALIDOMIDA TALIDOMIDA TALIDOMIDA ENSAIOS CLINICOS MIELOMA MULTIPLO MIELOMA MULTIPLO NEOPLASIAS NEOPLASIAS DEFEITOS DO TUBO NEURAL DEFEITOS DO TUBO NEURAL SENSIBILIDADE E ESPECIFICIDADE RELAÇAO ESTRUTURA-ATIVIDADE RESULTADO DE TRATAMENTO Location: BR191.1; 08505/s

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Id: 11607 Author: Williams, Diana L; Pittman, Tana L; Gillis, Thomas P; Matsuoka, Masanori; Kashiwabara, Yoshiko Title: Simultaneous detection of mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis ..- Source: s.l; s.n; 2001. 6 p. tab, graf. Descriptors: SEQUENCIA DE BASES DNA BACTERIANO DNA BACTERIANO DAPSONA RESISTENCIA MICROBIANA A DROGAS LEPROSTATICOS HANSENIASE DADOS DE SEQUENCIA MOLECULAR MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE ANALISE DE SEQUENCIA DE DNA ANALISE HETERODUPLEX/*MT Location: BR191.1; 08430/s

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Id: 11334 Author: Kirsztajn, G. M; Nishida, S. K; Silva, M. S; Lombardi, C; Ajzen, H; Pereira, A. B Title: Specific and nonspecific aspects of humoral immune response in leprosy ..- Source: s.l; s.n; 1994. 12 p. graf. Descriptors: ADOLESCENCIA ANTICORPOS ANTINUCLEARES ANTICORPOS ANTIBACTÉRIAS PROTEINA C-REATIVA CRIANÇA RELAÇOES HOSPEDEIRO-PARASITA HANSENIASE HANSENIASE HANSENIASE LEPROMATOSA HANSENIASE LEPROMATOSA MYCOBACTERIUM LEPRAE FATOR REUMATOIDE SENSIBILIDADE E ESPECIFICIDADE Location: BR191.1; 06946/s

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Id: 11318 Author: Rastogi, Nalin; Goh, Khye Seng; Berchel, Mylene Title: Species-specific identification of mycobacterium leprae by PCR-restriction fragment length polymorphism analysis of the hsp65 gene ..- Source: s.l; s.n; 1999. 4 p. ilus, tab, graf. Descriptors: ALGORITMOS ANTIGENOS DE BACTÉRIAS TATUS CHAPERONINAS GENES ESTRUTURAIS BACTERIANOS HANSENIASE FIGADO GANGLIOS LINFATICOS CAMUNDONGOS MYCOBACTERIUM LEPRAE MYCOBACTERIUM LEPRAE MYCOBACTERIUM TUBERCULOSIS MYCOBACTERIUM TUBERCULOSIS REAÇAO EM CADEIA POR POLIMERASE POLIMORFISMO DE FRAGMENTO DE RESTRIÇAO SENSIBILIDADE E ESPECIFICIDADE ESPECIFICIDADE DE ESPÉCIES BAÇO Location: BR191.1; 07257/s

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Id: 11304 Author: Kampirapap, Kowit Title: Mycobacterium leprae particle agglutination in diagnosis and monitoring of treatment of leprosy ..- Source: s.l; s.n; 1999. 5 p. tab, graf. Descriptors: ADOLESCENCIA TESTES DE AGLUTINAÇAO TESTES DE AGLUTINAÇAO ANALISE DE VARIANCIA ANTICORPOS ANTIBACTÉRIAS ANTIGENOS DE BACTÉRIAS CRIANÇA PRÉ-ESCOLAR MONITORAMENTO DE MEDICAMENTOS MONITORAMENTO DE MEDICAMENTOS GLICOLIPIDIOS LEPROSTATICOS HANSENIASE HANSENIASE MYCOBACTERIUM LEPRAE SENSIBILIDADE E ESPECIFICIDADE Location: BR191.1; 07243/s

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